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Image Search Results
Journal: Nature communications
Article Title: Cell atlas of the regenerating human liver after portal vein embolization.
doi: 10.1038/s41467-024-49236-7
Figure Lengend Snippet: Fig. 6 | Multiplexed immunohistochemistry reveals peri-vessel microenviron- ment alterations in post-PVE patient tissue sections. a Schematic illustrating the tissue-level 4i protocol. b DAPI staining of healthy liver tissue, illustrating the image resolution across length scales. Multiple staining overlays (expression of CLEC4M, ACTA2, IGFBP7, GLUL, Catalase representing pericentral LSECs, mesenchymal cells, periportal LSECs, hepatocytes, and peroxisomes, respectively) in a large healthy liver tissue section (c) and selected highlighted regions (d), where (i) shows anannotated liver lobule (P portal, C central), and the bottom panels a close-up of a portal (ii) and central (iii) vessel. Multiple staining overlays in large and highlighted sections of embolized (e) and regenerating (f) liver samples. g Portal and central vessel detection results from liver 4i data (Methods, Supplementary Fig. 16). Boxplots (bottom right) show normalized expression of ACTA2 per vessel and
Article Snippet: For the remaining analysis, the signal from the following antibodies was kept: CD45 (Abcam, ab10558),
Techniques: Immunohistochemistry, Staining, Expressing